University of Veterinary Medicine Hannover, Foundation Clinic for Horses |
Discover our other app, the OGT Reference Provider with a dynamic
reference range (90 to 180 min) for the oral glucose test, providing a more practicable test for
insulin dysregulation! → Visit ogt-reference-provider.org! |
As demonstrated in figure 2, insulin cut-offs are not applicable as-is, independently of the assay used, warranting the use of assay-specific reference ranges or, in their absence, approximate solutions such as our conversion app.
The conversion of insulin measurements is not strictly proportional to an assay-specific coefficient. The interaction between assays and insulin ought to be investigated using recombinant equine insulin or standardised pooled samples available to all laboratories.
A paper describing the exact methodology to obtain the conversion functions was recently published. Briefly, monotonically non-decreasing splines were fitted with non-negative least-squares regression to describe the relationship between assay pairs, as derived from samples where insulin was measured with two or more assays concurrently. Each function was visually inspected to preclude overfitting. The obtained functions were transformed to polynomials and embedded in the present app. The intervals given on the right hand side before any conversion are the ranges for which data is available in the app. After conversion, prediction intervals are given instead. They describe the uncertainty around the conversion which depends on the amount of available data and concordance of the assay pairs.
All the information on this website is published in good faith and for general information purpose only. We do not make any warranties about the completeness, reliability and accuracy of this information. The results provided by the conversion module may change in future versions at the sole discretion of the authors. Any action you take upon the information you find on this website, is strictly at your own risk.